The binding profile of newly isolated anti-poliovirus antibodies A1, E10, F12, and G10. Each of the three serotypes of OPV (types 1, 2, and 3 represented by red, yellow, and green, respectively) at a concentration of 5 × 106 pfu/mL was captured on a 96-well ELISA plate coated with purified rabbit polyclonal serotype-specific IgG (2 µg/mL) and was reacted with phage-displayed Fabs (A) or with soluble Fab (B) at a concentration of 3 µg/mL. The binding was measured by ELISA. OD, optical density measured at 450 nm.