Table 1.

Functional affinities of anti-poliovirus A12 IgG

Poliovirus serotype	koff, 1/s	kon, 1/Ms	Kd, M
1	1.87 × 10−4	6.68 × 104	2.8 × 10−9
2	1.62 × 10−4	5.54 × 104	2.92 × 10−9
3	2.91 × 10−4	9.28 × 103	3.61 × 10−8

Binding kinetics of A12 IgG to poliovirus particle was assessed by SPR using a Biacore 1000. The kinetic rate constants of association (kon) and dissociation (koff) were measured from surface binding kinetics, and the equilibrium dissociation constant (Kd) was calculated as the ratio koff/kon.