Fig. 3.

Gene expression validation of HPAT. (A) Gene expression analysis by qPCR was performed on two hESC samples (H1 and H9), one iPSC line (RIPSC.HUF1), and a collection of cDNAs from fetal and adult tissues. (B) Gene expression profiling of HPAT genes in single blastomeres of eight-cell embryos and blastocysts. E1 and E2 denote the embryos from which blastomeres were isolated. (C) Reactivation of genes during the reprogramming process. Cells were analyzed at different time points of mRNA-mediated iPSCs derivation.