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Functional effect of down-regulation of HPAT genes. Gene expression analysis by qPCR of different pluripotency-associated genes after down-regulation of GFP (negative control, in green), OCT4 (positive control, in yellow), and HPAT1 (in red). siRNAs for GFP and HPAT1 were derived by in vitro dicer-mediated digestion of the corresponding double-stranded mRNAs; siRNAs for OCT4 were designed in silico. Analysis was performed 24 h after the transfection.
