Fig. 2.

Structure–activity relationship for EGA. EGA and commercially available structural analogs were tested for ability to inhibit LT-mediated cytotoxicity. RAW264.7 cells were seeded at 2 × 10³ cells/well on 384-well plates and the following day were incubated with indicated doses of (A) EGA [4-bromobenzaldehyde N-(2,6-dimethylphenyl)semicarbazone] (5319257), (B) Benzaldehyde N-(2,6-dimethylphenyl)semicarbazone (5319438), (C) N-(2,6-dimethylphenyl)-N′-(4-fluorophenyl)urea (9062150), and (D) 4-chlorobenzaldehyde N-phenylsemicarbazone (5191420) for 1 h, followed by intoxication with 500 ng/mL PA + 500 ng/mL LF. Cell viability was measured 24 h later as in Fig. 1. Average values ±SD from technical replicates in a single representative experiment shown.