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. 2013 Nov 25;110(50):20326–20331. doi: 10.1073/pnas.1314336110

Fig. 3.

Fig. 3.

UVR8W285A expression results in a constitutive photomorphogenic response. (A and B) Analysis of hypocotyl length and quantification of 4-d-old seedlings grown in the dark (A) or in continuous white light (3.6 µmol⋅m−2⋅s−1) (B). Histograms show average hypocotyl length; error bars represent SD (n > 30). (Scale bar, 5 mm.) (C) Quantitative RT-PCR analysis of COP1 mRNA levels in 4-d-old UVR8W285A transgenic seedlings under white light compared with wild-type seedlings (Ws). (D) Immunoprecipitation and Western blot analysis of COP1 protein levels in 7-d-old seedlings grown in white light (−UV-B) or supplemented for 6 h with narrowband UV-B before harvesting (+UV-B). (E and F) Quantitative RT-PCR comparison of RUP2 and ELIP2 mRNA levels in 4-d-old wild-type (Ws) and UVR8W285A transgenic plants grown in white light. (G and H) Quantitative RT-PCR comparison of HY5 mRNA levels in 4-d-old wild-type (Ws) and UVR8W285A transgenic plants grown in the dark (G) or in white light (H). (I and J) Immunoblot analysis of HY5 protein levels in 7-d-old seedlings grown in the dark (I) or in white light (J). Asterisks indicate unspecific bands.