Fig. 4.
UVR8W285A expression results in elevated constitutive levels of chalcone synthase and anthocyanins as well as elevated UV-B tolerance. (A and B) Analysis of CHS mRNA levels (A) and CHS protein levels (B) in 4-d-old seedlings grown in white light. (C) Photometric determination of the anthocyanin content of 4-d-old Arabidopsis seedlings. Data shown are the mean values of three independent biological replicates; error bars indicate SD. Representative images showing the elevated anthocyanin content (purple pigmentation) in a 4-d-old transgenic plant overexpressing UVR8W285A (Right). (D) UV-B tolerance of non–UV-B–acclimated 7-d-old seedlings treated for 3 h with broadband UV-B. After UV-B stress treatment, the seedlings were allowed to recover for 1 wk without UV-B before the image was captured.