Fig. 6.

UVR8/UVR8^W285A “interactors” include SPA family proteins, but interaction with SPA1 is indirect via COP1. (A) Proteins identified after TAP purification with GS-TAP-UVR8^W285A expressed in Arabidopsis cell-suspension cultures [PSB-L/PKSA::GS(rhino)-UVR8^W285A]. Known TAP background proteins were filtered from the list. AGI, Arabidopsis Genome Initiative identifier. See Table S1 for details of protein identification. (B) Coimmunoprecipitation of COP1 and SPA1 using UVR8 antibodies in extracts from 7-d-old wild-type (Col), cop1-4, and spa1-3 seedlings. Seedlings were irradiated for 6 h with supplemental narrowband UV-B and for 15 min with (+) or without (−) broadband UV-B.