Fig. 2.

Cep152 contains a binding motif analogous to that of Cep192, and competes against the latter in Plk4 binding. (A and B) 293T cells cotransfected with the indicated constructs were analyzed. (C) 293T cells expressing FLAG₃-Plk4, HA-Cep192, or increasing amounts (black right-angled triangle) of HA-Cep152 were mixed, as indicated (plus symbols), and subjected to coimmunoprecipitation analyses. Numbers indicate relative signal intensities. (D) For Cep152 and Cep192 expression, 293T cells were singly transfected or cotransfected with the indicated FLAG- or GFP-tagged constructs. The resulting total lysates expressing Cep192, Cep152, or both were mixed with the same amounts of other total lysates expressing either control vector or FLAG₃-Plk4, and then the combined lysates were subjected to coimmunoprecipitation analyses. Numbers indicate relative expression levels in the input and amounts of coimmunoprecipitated proteins. The graph shows calculated levels of interactions based on the signal intensities quantified from the blots.