Fig. 3.

Expression of Cep192 DN80 or Cep152 DN217 is sufficient to alter subcellular localization of Plk4. (A) 293T cells transfected with the indicated constructs were subjected to GST pull-down assays. Arrowheads mark endogenous Plk4 coprecipitated with GST-DN80 or GST-DN217, but not with their respective 2A mutant or control GST (not shown because of small molecular size). (B and C) U2OS cells stably expressing the indicated GFP-DN80 or GFP-DN217 constructs were subjected to immunoblotting analyses (B) using antibodies that detect the epitope commonly present in the GFP-fused form and its respective endogenous protein. The cells were also immunostained (C) to determine the level of Plk4 at the centrosomes. Asterisks in (B) mark nonspecific cross-reacting proteins.