Fig. 5.

CD8⁺ T-cell activation is increased at TT. (A) The lymphocyte population identified in single-cell suspensions of 4T1 tumors (average volume: ST = 737.5 ± 117.6 mm³; TT = 394.2 ± 35.69 mm³) was gated on forward and side scatter. The percentage of lymphocytes that were (B) CD3⁺CD8⁺, (C) CD69⁺CD3⁺CD8⁺, (D) IFN-γ–producing CD3⁺CD8⁺, and (E) Glut-1–expressing CD3⁺CD8⁺ were measured. Data presented as mean ± SEM (n = 4–5; *P < 0.05; Student t test).