Fig. 5.

Highly enriched Notch1 LoF mutation in LGK974-responsive HNSCC cell lines. (A) Diagrams of potential LoF mutations of Notch1 in HNSCC cell lines. C, C terminus; LNR, Lin12-Notch repeat; N, N terminus; PEST, proline, glutamic acid, serine, threonine-rich; TMD, transmembrane domain. Frameshift (FS) and nonsense mutations (X), including E488fs, A495fs, K538fs, G192X, P460fs, and E216X, are highlighted in red. (B) The list of Notch1 FS and nonsense mutations in HNSCC cell lines. (C) Notch1 C478F showed complete reduction of activities compared with the WT in a Notch1 reporter gene assay with or without DLL1 stimulation.