Figure 1.

I-kappa-B-alpha degradation and IKKalpha/beta phosphorylation analysis in response to anti-IgM in B-1a cells. Sorted peritoneal B-1a and splenic B-2 cells were treated with anti-IgM (15 μg/ml) for the times indicated at 37°C. Afterward, the cells were washed, pelleted, lysed, and then used for western blot analysis. Results shown are representative of three independent experiments.