Table 1.
Primer name | Sequence (5′-3′ orientation) |
---|---|
MiR-34a- RT primer | 5′-GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA CAA CC-3′ |
MiR-34a mimics | 5′- TGG CAG TGT CTT AGC TGG TTG T -3′ |
Mimics control | 5′-AGT GTG AGT TCT ACC ATT GCC AAA-3′ |
MiR-34a inhibitor | 5′-ACA ACC AGC TAA GAC ACT GCC A-3′ (2′Ome) |
MiR-34a -F | 5′-CGG GCT GGC AGT GTC TT-3′ |
MiR-34a -R | 5′-GTG CAG GGT CCG AGG T-3′ |
MiR-223 | 5′-TGT CAG TTT CTC AAA TAA ACC CCA-3′ |
RNU6- RT | 5′-AAA ATA TGG AAC GCT-3′ |
RNU6-F | 5′-CTC GCT TCG GCA GCA CA-3′ |
RNU6-R | 5′-AAC GCT TCA CGA ATT TGC GT-3′ |
GAPDH-F | 5′-AGG GCA TCT TGG GCT ACA C-3′ |
GAPDH-R | 5′-TGG TCC AGG GTT TCT TAC TCC-3′ |
lmtk3-F4172 | 5′-TCG ACC AGG AGA CGC C-3′ |
lmtk3-R4329 | 5′-CTC GAA ACT GCC TCC AAA G-3′ |
LMTK3-3'UTR-F-Xhol | 5′-AAA ACT CGA GAT TCC CCG AAG ACC CG-3′ |
LMTK3-3'UTR-R-Not I | 5′-AAA AGC GGC CGC TTC CTC CAA AAA GTT GTT-3′ |
LMTK3-CDS-F-Hind III | 5′-CCC CAA GCT TAT GAG GCA AGT GCT GTG G-3′ |
LMTK3-CDS-R-EcoR I | 5′-AAA AGA ATT CTC AAT TCT CCA CGG GG-3′ |
The underlined letters are the endonuclease sites and the bases before these sites are protection bases.
RT, reverse transcription.