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. 2013 Dec;32(12):699–707. doi: 10.1089/dna.2013.2130

Table 1.

Information on the Primers Used for Reverse Transcription, Real-Time Polymerase Chain Reaction, and Construction of Plasmids

Primer name Sequence (5′-3′ orientation)
MiR-34a- RT primer 5′-GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA CAA CC-3′
MiR-34a mimics 5′- TGG CAG TGT CTT AGC TGG TTG T -3′
Mimics control 5′-AGT GTG AGT TCT ACC ATT GCC AAA-3′
MiR-34a inhibitor 5′-ACA ACC AGC TAA GAC ACT GCC A-3′ (2′Ome)
MiR-34a -F 5′-CGG GCT GGC AGT GTC TT-3′
MiR-34a -R 5′-GTG CAG GGT CCG AGG T-3′
MiR-223 5′-TGT CAG TTT CTC AAA TAA ACC CCA-3′
RNU6- RT 5′-AAA ATA TGG AAC GCT-3′
RNU6-F 5′-CTC GCT TCG GCA GCA CA-3′
RNU6-R 5′-AAC GCT TCA CGA ATT TGC GT-3′
GAPDH-F 5′-AGG GCA TCT TGG GCT ACA C-3′
GAPDH-R 5′-TGG TCC AGG GTT TCT TAC TCC-3′
lmtk3-F4172 5′-TCG ACC AGG AGA CGC C-3′
lmtk3-R4329 5′-CTC GAA ACT GCC TCC AAA G-3′
LMTK3-3'UTR-F-Xhol 5′-AAA ACT CGA GAT TCC CCG AAG ACC CG-3′
LMTK3-3'UTR-R-Not I 5′-AAA AGC GGC CGC TTC CTC CAA AAA GTT GTT-3′
LMTK3-CDS-F-Hind III 5′-CCC CAA GCT TAT GAG GCA AGT GCT GTG G-3′
LMTK3-CDS-R-EcoR I 5′-AAA AGA ATT CTC AAT TCT CCA CGG GG-3′

The underlined letters are the endonuclease sites and the bases before these sites are protection bases.

RT, reverse transcription.