Table 2.
Effect of plasmids carrying mutations in 67RuvC on the survival of UV-irradiated ruvABC and ruv+ E. coli strains
| Strain | Fraction surviving (30 J m−2) | |
|---|---|---|
| N4454 | AB1157 | |
| Genotype | ΔruvABC::cat | ruvABC+ |
| wt | 0.60 ± 0.005 | 0.62 ± 0.015 |
| S10A | 0.61 ± 0.010 | 0.49 ± 0.030 |
| T11A | 0.0023 ± 0.0012 | 0.69 ± 0.015 |
| K40A | 0.67 ± 0.010 | 0.79 ± 0.030 |
| R46A | 0.0026 ± 0.00015 | 0.80 ± 0.015 |
| S109A | 0.46 ± 0.0050 | 0.60 ± 0.075 |
| K110A | 0.52 ± 0.0050 | 0.78 ± 0.050 |
| K120A | 0.50 ± 0.0010 | 0.62 ± 0.115 |
| R121A | 0.068 ± 0.022 | 0.71 ± 0.012 |
| R124A | 0.0014 ± 0.00025 | 0.81 ± 0.050 |
| K125A | 0.0014 ± 0.0011 | 0.41 ± 0.060 |
| R124A+K125A | 0.0014 ± 0.00005 | 0.80 ± 0.025 |
| pET24a | 0.0016 ± 0.00020 | 0.66 ± 0.040 |
bIL67 ruvC and mutant derivatives cloned in pET24a(+) and relevant strain genotypes are indicated. Results are the mean and standard deviation of at least two independent experiments.