Figure 2.

Arabinose‐regulated activity of the integrated SssI methylase gene in E. coli.
A. Transformants were grown in LB medium containing kanamycin and arabinose 0.2% and bacterial genomic DNA was isolated. The extent of CpG methylation was determined by cutting with the restriction endonuclease HpaII and evaluating the extent of DNA cleavage. Comparisons were made with uncut genomic DNA of the same samples. Control bacterial DNA came from non‐transformed Top10 cells. The arrowhead points to the location of high‐molecular‐weight genomic DNA fragments.
B. Transformants were electroporated with the plasmid pGreenLantern and grown in LB containing kanamycin and ampicillin with either 0.2% glucose, no sugar or arabinose at varying percentages (w/v). After overnight culture, mini‐prep DNA was cut with HpaII to analyse the methylation status of each plasmid. The arrowhead points to the location of uncut supercoiled plasmid DNA.
L is a DNA ladder lane.