Figure 5.

Transposition of methylated SB transposon. HeLa cells were transiently transfected with unmethylated (Unmeth) or in vivo methylated (Meth In vivo) neomycin‐resistant transposon DNA from the Sleeping Beauty transposon together with unmethylated plasmid expressing the transposase (pCMV‐HSB16). One set of cells received unmethylated transposon and no transposase (No Tase). Two days after transfection, cells are plated at low density and selected in drug for 2 weeks to generate colonies. The mean number of colonies from four or more independent experiments is graphed. A significant difference was observed showing increased transposition following methylation (*P < 0.003).