Table 1.
Genes of four hydrogenase operons (hya, hyb, hyc, hyf) in E. coli.
| Gene | Size, bp | Description |
|---|---|---|
| hyaA | 372 | Hydrogenase 1, small subunit |
| hyaB | 597 | Hydrogenase 1, large subunit |
| hyaC | 235 | Hydrogenase 1, b‐type cytochrome subunit |
| hyaD | 195 | Protein involved in processing of HyaA and HyaB proteins |
| hyaE | 132 | Protein involved in processing of HyaA and HyaB proteins |
| hyaF | 285 | Protein involved in nickel incorporation into hydrogenase‐1 proteins |
| hyfA | 205 | Hydrogenase 4, 4Fe‐4S subunit |
| hyfB | 672 | Hydrogenase 4, membrane subunit |
| hyfC | 315 | Hydrogenase 4, membrane subunit |
| hyfD | 479 | Hydrogenase 4, membrane subunit |
| hyfE | 216 | Hydrogenase 4, membrane subunit |
| hyfF | 526 | Hydrogenase 4, membrane subunit |
| hyfG | 555 | Hydrogenase 4, subunit |
| hyfH | 181 | Hydrogenase 4, Fe‐S subunit |
| hyfI | 252 | Hydrogenase 4, Fe‐S subunit |
| hyfJ | 137 | Predicted processing element hydrogenase |
| hyfR | 670 | DNA‐binding transcriptional activator, formate sensing |
| hycI | 156 | Protease involved in processing C‐terminal end of HycE |
| hycH | 136 | Protein required for maturation of hydrogenase 3 |
| hycG | 255 | Hydrogenase 3, small subunit |
| hycF | 180 | Formate hydrogenlyase complex Fe‐S protein |
| hycE | 569 | Hydrogenase 3, large subunit |
| hycD | 307 | Hydrogenase 3, membrane subunit |
| hycC | 608 | Hydrogenase 3, membrane subunit |
| hycB | 203 | Hydrogenase 3, Fe‐S subunit |
| hycA | 153 | Regulator of the transcriptional regulator FhlA |
| hybG | 82 | Hydrogenase 2 accessory protein |
| hybF | 113 | Protein involved with the maturation of hydrogenases 1 and 2 |
| hybE | 162 | Hydrogenase 2‐specific chaperone |
| hybD | 164 | Predicted maturation element for hydrogenase 2 |
| hybC | 567 | Hydrogenase 2, large subunit |
| hybB | 392 | Predicted hydrogenase 2 cytochrome b‐type component |
| hybA | 328 | Hydrogenase 2 4Fe‐4S ferredoxin‐type component |
| hybO | 372 | Hydrogenase 2, small subunit |