Table 2.

Identity between hydrogenase large subunits derived from Escherichia coli (E. coli), Ralstonia eutropha H16 (R. eutropha), Rhodoccus opacus MR11 (R. opacus), Synechocystis sp. PCC 6803 (Syn. PCC 6803) and Desulfovibrio gigas (D. gigas).

	E. coli Hyd‐1	E. coli Hyd‐2	E. coli Hyd‐3	E. coli Hyd‐4	R. eutropha HoxH	R. opacus HoxH	Syn. PCC 6803 HoxH	D. gigas large subunit
E. coli Hyd‐1		40.7%	13.9%	11.9%	18.8%	18.3%	16.1%	39.9%
E. coli Hyd‐2	40.7%		13.8%	11.0%	17.3%	18.1%	19.0%	41.6%
E. coli Hyd‐3	13.9%	13.8%		69.8%	13.1%	12.2%	12.4%	13.7%
E. coli Hyd‐4	11.9%	11.0%	69.8%		12.7%	12.0%	13.0%	12.4%
R. eutropha HoxH	18.8%	17.3%	13.1%	12.7%		85.2%	42.7%	19.9%
R. opacus HoxH	18.3%	18.1%	12.2%	12.0%	85.2%		42.0%	19.8%
Syn. PCC 6803 HoxH	16.1%	19.0%	12.4%	13.0%	42.7%	42.0%		17.8%
D. gigas large subunit	39.9%	41.6%	13.7%	12.4%	19.9%	19.8%	17.8%

Hyd‐1, Hyd‐2, Hyd‐3, Hyd‐4 are HyaB, HybC, HycE and HyfG of E. coli hydrogenases 1, 2, 3 and 4 respectively. Protein sequences for the large subunit or HoxH of hydrogenase were aligned by using a VectroNTI alignment software.