Table 3.
Blockade of Methoxsalen’s Effects on Nicotine Acute Pharmacological Responses After Oral Administration in Mice
| Treatment (mg/kg) | Tail-flick test % MPE | Hot-plate test % MPE | Hypothermia (∆˚C) (mean ± SEM) |
|---|---|---|---|
| Saline/vehicle/saline | 6±2 | 7±3 | −0.1±0.3 |
| Saline/methoxsalen (15)/saline | 8±3 | 11±6 | −1.5±0.3* |
| Meca (2)/methoxsalen (15)/saline | 7±5 | 12±7 | −1.2±0.6 |
| Meca (2)/vehicle/saline | 8±3 | 6±4 | −0.5±0.3 |
| Meca (2)/vehicle /nicotine (15) | 1±1 | 6±3 | −0.5±0.1 |
| Saline/vehicle /nicotine (15) | 5±1 | 10±5 | −1.6±0.2* |
| Saline/methoxsalen (15)/nicotine (2.5) | 87±9*,# | 65±8*,# | −5.8±0.2*,# |
| Meca (2)/methoxsalen (15)/nicotine (2.5) | 7±3 | 14±6 | −1.9±0.3* |
Note. Animals were pretreated with either saline or mecamylamine (2mg/kg, sc) followed by methoxsalen (15mg/kg, ip). Thirty minute later, mice received nicotine at a dose of 15mg/kg (po) and were then tested at 5 and 30min after injection for antinociception and hypothermia, respectively. Methoxsalen (15) = methoxsalen at 15mg/kg, ip; meca (2) = mecamylamine at 2mg/kg, sc; nicotine (15) = nicotine at 15mg/kg, po. Each point represents the mean ± SE of 8–12 mice.
*p < .05 compared with Saline/Vehicle/Saline.
# p < .05 compared with Saline/methoxsalen (15)/Saline.