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. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Mol Biochem Parasitol. 2013 Mar 21;188(2):10.1016/j.molbiopara.2013.02.004. doi: 10.1016/j.molbiopara.2013.02.004

Fig 6 — Recording technique used for the pharynx to record the conductance changes produced in response to cumulative applications of abamectin and derquantel in pharyngeal preparations. (A) Diagram of the technique. Positioning of: I, current injecting electrode and; V, voltage recording electrode in the terminal bulb of pharynx. P, perfusion needle above the pharynx for a localized perfusion of calcium free APF Ringer and or drugs E, the earth electrode to complete the circuit. The cumulative applications of abamectin (0.01 to 1 μM) and derquantel (0.1 to 10 μM). (B) Representative trace showing the conductance changes produced in response to cumulative applications of abamectin (C) Representative trace showing the conductance changes produced by the cumulative applications of derquantel. (D) The concentration conductance plots fitted with non-linear regression for abamectin and derquantel . The EC₅₀ for abamectin was 400 nM (n = 4). It was not possible to fit non-linear regression for derquantel due to a little or no conductance change produced by derquantel (one sample t-test, n = 4).

Inline graphic — Recording technique used for the pharynx to record the conductance changes produced in response to cumulative applications of abamectin and derquantel in pharyngeal preparations. (A) Diagram of the technique. Positioning of: I, current injecting electrode and; V, voltage recording electrode in the terminal bulb of pharynx. P, perfusion needle above the pharynx for a localized perfusion of calcium free APF Ringer and or drugs E, the earth electrode to complete the circuit. The cumulative applications of abamectin (0.01 to 1 μM) and derquantel (0.1 to 10 μM). (B) Representative trace showing the conductance changes produced in response to cumulative applications of abamectin (C) Representative trace showing the conductance changes produced by the cumulative applications of derquantel. (D) The concentration conductance plots fitted with non-linear regression for abamectin and derquantel . The EC₅₀ for abamectin was 400 nM (n = 4). It was not possible to fit non-linear regression for derquantel due to a little or no conductance change produced by derquantel (one sample t-test, n = 4).