Table 1.

Summary of NMR structure statistics and restraints for the hGlyR-α1 TM domain in LPPG micelles. See also Figures S3, S4, and S5.

NMR Distance & Dihedral Restraints	Monomer	Pentamer
Distance restraints
Total NOE	1014	1014 × 5
Intra-residue	321	321 × 5
Inter-residue	693	693 × 5
Sequential (|i-j| = 1)	348	348 × 5
Medium-range (|i-j| ≤ 4)	324	324 × 5
Long-range (|i-j| > 4)	21	21 × 5
Hydrogen bonds	106	106 × 5
Total dihedral angle restraints	152	154 × 5
Phi	76	77 × 5
Psi	76	77 × 5
PRE restraints	219	226 × 5
Upper	107	114 × 5
Lower	112	112 × 5
Inter-subunit distance restraints from EM and RDCa
Total constraints		600
Upper		300
Lower		300
Structure Statistics
Violations (mean and s.d.)
Upper distance restraints (Å)	0.0075 ± 0.0008	0.0157 ± 0.0010
Lower distance restraints (Å)	0.0016 ± 0.0010	0.0102 ± 0.0012
Dihedral angle restraints (°)	0.130 ± 0.010	0.338 ± 0.028
Max. dihedral angle violation (°)	1.29	3.32
Max. distance restraint violation (Å)	0.24	0.63
Average pairwise r.m.s.d.b (Å)
Heavy	1.04 ± 0.11c	0.91 ± 0.14c
	2.27 ± 0.25d	1.50 ± 0.36d
Backbone	0.67 ± 0.13c	0.50 ± 0.17c
	1.66 ± 0.18d	0.95 ± 0.30d
Ramachandran Plot
Residues in most favored regions	88.6%	86.3%
Residues in allowed regions	11.3%	13.0%
Residues in disallowed regions	0.1%	0.7%
PDB code	2M6B	2M6I

^aRDC values used to generate pentamer restraints were obtained from the previous study (Canlas et al., 2008).

^bThe rmsd to the average coordinates was calculated from 15 structures.

^cCalculated over the helical TM regions (residues 220-241, 250-270, 289-305, 398-421)

^dCalculated over residues 215-425