Skip to main content
. 2011 Jul 20;31(29):10529–10539. doi: 10.1523/JNEUROSCI.0784-11.2011

Figure 1.

Figure 1.

Halo is specifically expressed in orexin neurons in the orexin/Halo transgenic mouse brain. A, Orexin-IR neurons are located in the LHA (Alexa Fluor 594, red). GFP-IR neurons, indicating Halo expression, are also observed in the LHA (Alexa Fluor 488, green). Merged picture shows specific expression of Halo in orexin neurons in the LHA in the orexin/Halo transgenic mice (top row). Scale bar, 100 μm. More than 90% of orexin neurons express Halo. GFP-IR is observed in the soma and dendrites. No expression of Halo other than in orexin neurons was observed throughout the brain. Bottom row is higher magnifications of square region in the top row. Scale bar, 50 μm. B, Confocal microscopic image of a 15-week-old orexin/Halo transgenic mouse showing that Halo expression is observed in the soma and dendrites of orexin neurons (arrows) but not in axons (arrowhead). Scale bar, 50 μm.