Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Exp Eye Res. 2013 Oct 9;116:10.1016/j.exer.2013.09.019. doi: 10.1016/j.exer.2013.09.019

Fig. 3.

Fig. 3

Fig. 3

Localization and colocalization of WT-AQP0-mCherry and AQP0-R33C-EGFP. (a, b) Epifluorescent images of MDCK cells transfected with the WT-AQP0-mCherry and AQP0-R33C-EGFP, respectively. c. Western blot analysis of MDCK cells expressing WT-AQP0-mCherry (lane 1) and AQP0-R33C-EGFP (lane 2). (d–f) Cells cotransfected with WT-AQP0-mCherry and AQP0-R33C-EGFP constructs; (d) cotransfected cell viewed under mCherry fluorescent filter; (e) the same cells under an EGFP fluorescent filter; (f) overlaid image of (d) and (e).