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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Biotechnol Prog. 2013 Oct 9;29(6):10.1002/btpr.1810. doi: 10.1002/btpr.1810

Figure 2. Purification of Recombinant mfp-6.1.

Figure 2

Purification of recombinant mfp-6.1. (A) 15% SDS-PAGE with Coomassie blue staining of the His6-tag purification of rmfp-6.1 using fast protein liquid chromatography and (B) MALDI TOF mass spectrum of the MFPLC-purified rmfp-6.1. Matrix=α-cyano-4-hydroxycinnamic acid, Accelerating Voltage=25000 V, Grid Voltage=93%, Guide Wire Voltage = 0.3, Delay Time=300ms, CE = crude extract, El2-4 = flow through, El5-8 = elution, M = marker. Please refer to the FPLC chromatogram in Figure S9 for more details.