Figure 4.
Concentration-dependent effects of acetyl-L-carnitine (ALCAR) on the respiratory capacity of DIV 14 and 7 rat cortical neurons in the presence of glucose. Experiments were performed as described in Figure 1. (A) Effects of ALCAR on uncoupled OCR obtained with DIV 14 neurons. (B) Effects of ALCAR on uncoupled OCR obtained with DIV 7 neurons. (C) Effects of transient excitotoxic glutamate receptor stimulation and ALCAR on uncoupled OCR obtained with DIV 14 neurons. (D) Effects of transient excitotoxic glutamate receptor stimulation and ALCAR on uncoupled OCR obtained with DIV 7 neurons. Values for OCR obtained after FCCP addition are normalized as in Figure 2. Values are expressed as means ± s.e. for n = 3–6 different neuronal preparations. * p < 0.05 compared to the absence of glutamate (C and D). # p < 0.05 compared to the absence of ALCAR following exposure to glutamate (D).
