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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biotechniques. 2013 Jul;55(1):10.2144/000114056. doi: 10.2144/000114056

Figure 4. Counting of dense confluent cell layers.

Figure 4

SHEP-GFP cells were seeded at very high cell densities of ~5000k / well and allowed to grow for one day, resulting in a fully confluent cell layer. Cells were imaged with fluorescence, pinhole, and pinhole + PBS techniques. Cell counts of pinhole-illuminated images with and without PBS incubation were normalized against fluorescence images of the same viewing field (means ± SD, n = 6).