Figure 2. MiR-129-5p can be induced by IFN-β in Hela cells.

(A) Validation of the microarray data by qPCR. Triplicate assays were performed for each sample. Inductions of known IFN-regulated genes ISG54 and ISG56 are shown for comparsion. (B) Time course of miRNA induction by IFN-β. Hela cells were stimulated with 300 IU ml⁻¹ IFN-β for the indicated times, and miR-129-5p and ISG54/ISG56 expressions were quantified by qPCR. (C) Dose-response analysis of miRNA induction by IFN-β. Hela cells were stimulated with the indicated doses of IFN-β for 2 h, and miR-129-5p and ISG54/ISG56 expressions were quantified by qPCR.