Table 2. Presence of Bluetongue infectious virus or RNA in foetal tissues: BTV 8 infected group+commingled control.

		Foetal tissues
		Umbilical cord blooda		Spleen	Cerebrum	Cerebellum
Group	Foetus number	VI	RT-qPCR	VI	VI	VI
Uninfected controls	78600.1	−	46.00	−	−	−
BTV8 infected	5572.1	−	46.00	−
	5572.2	−	46.00	−
	5572.3	−	40.75	−
	5572.4	−	46.00	−
	78612.1	+	16.20	+	+	+
	78612.2	−	46.00	−
	78613.1	−	42.52	−
	78613.2	n.s.	n.s.	−
	78613.3	n.s.	n.s.	−
	78618.1	−	46.00	−
	78648.1	−	19.94	+	−	+
	78648.2	+	21.57	+	+	+
	78648.3	+	20.54	+	+	+
	78648.4	+	23.88	+	+	+
	78653.1	−	46.00	−
	78653.2	−	46.00	−
	78653.3	−	42.03	−
	78653.4	−	41.72	−
	78653.5	−	46.00	−
	78654.1	+	16.80	+	+	+
	78654.2	+	17.08	+	+	+
	78654.3	+	27.48	+	−	−
	78654.4	+	22.76	+	−	+
	78676.1	+	18.06	+	+	+
	78676.2	+	16.27	+	+	+
	78676.3	+	18.30	+	+	+
	78678.1	−	46.00	−
	78678.2	−	46.00	−
Total no. of pos samples/no. of samples tested		11/24	12/26	12/28	9/12	11/12

 = positive (in bold), 30≤Ct<35 = doubtful, Ct≥35 = negative.^a RT-qPCR result: Ct<30

Virus isolation (VI): + cpe detected; −: no cpe detected. ns: no sample.