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. 2013 Dec 16;8(12):e81774. doi: 10.1371/journal.pone.0081774

Figure 3. Treatment of M1 macrophages with IL-4 did not result in upregulation of miR-124 and conversion of M1 cells into M2.

Figure 3

Peritoneal CD11b+F4/80+ macrophages were isolated and left unpolarized (M0) or polarized towards M1, or M2, or first polarized towards M1, and after 24 hours, washed and polarized towards M2 (M1→M2 or M/M2) as described in Materials and Methods. After which, the cells were stained for CD206, CD86 and MHC class II and analyzed by FACS (A), or used for RNA isolation and analysis of expression of miR-155 (B) or miR-124 (C) by real-time RT-PCR. One representative experiment of three is shown in (A–C); mean ± S.E. of triplicate is shown in (B, C).