Skip to main content
. Author manuscript; available in PMC: 2013 Dec 16.
Published in final edited form as: Sci Transl Med. 2012 Oct 10;4(155):10.1126/scitranslmed.3004371. doi: 10.1126/scitranslmed.3004371

Fig. 2.

Fig. 2

HuCNS-SCs preferentially differentiate into oligodendrocytes. (A and B) Confocal images demonstrate the myelination of axons by human cells 12 weeks after transplant in neonatal mice. (A) In cerebellum, a SC121+ human cell (red) coexpressing Olig2+ (green) appeared contiguous with several MBP+ myelin sheaths (light blue). Dark blue, Hoechst nuclear counterstain. (B) The mature oligodendrocyte marker CC1 (green) is coexpressed with SC121 (red) in the corpus callosum and demonstrates the typical morphology of a human myelinating cell. Note the diffuse colocalization of CC1 and SC121 in the cytoplasm (yellow-orange). (C to F) Cell fate analysis of human cells 10 weeks after neonatal Shi-id transplantation. Representative immunohistochemical staining in the cerebellum of neonatal Shi-id mice is shown for the human marker SC121 (C), MBP (D), human nestin (hNestin) (E), and human GFAP (hGFAP) (F). Arrowheads indicate immunoperoxidase staining of human markers. Regional boundaries are delineated with methyl green counterstain.