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. 2013 Dec 10;8:1–11. doi: 10.2147/DDDT.S52714

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© 2014 Li et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution — Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Gene expression profile of RAW 264.7 cells following polymethylmethacrylate stimulation and naringin treatment.

Notes: RAW 264.7 cells were preincubated with or without naringin (10 μg/mL) for 3 hours prior to the addition of 1% polymethylmethacrylate (PMMA) particles. After treatment for 6 hours, total RNA was isolated and gene expression was evaluated by real time polymerase chain reaction techniques. RAW 264.7 cells cultured in medium without naringin, vehicle, or particles were used as control. Data were collected in triplicate cultures of RAW 264.7 cells from three independent experiments. *Denotes P<0.05 when compared with the PMMA group; ^#denotes P<0.05 when compared with the vehicle + PMMA group.

Abbreviations: CPK, cathepsin K; PMMA, polymethylmethacrylate; RANK, receptor activator of nuclear factor-kappa B; TRAP, tartrate resistant acid phosphatase; mRNA, messenger RNA.