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. 2013 Aug 27;7(6):1116–1128. doi: 10.1016/j.molonc.2013.08.007

Figure 2.

Figure 2

Increase of c‐Ski promotes activation of breast NFs into CAFs. (A) Levels of c‐Ski and α‐SMA were determined by Western blot analysis. Higher levels of c‐Ski and α‐SMA were found in CAFs than in NFs. The data represent means ± s.d. from three different experiments. (*P < 0.01, Student t test). (B) c‐Ski expression in CAFs and NFs detected by immunofluorescence. Green: c‐Ski; Blue: DAPI. Scale bars, 200 μm. (C) Western blot analysis of c‐Ski, α‐SMA and FAP in parental NFs, parental CAFs, NFs with c‐Ski overexpression and corresponding control cells (Upper panel). β‐Actin is loading control. The relative fold change of c‐Ski, α‐SMA and FAP in cells is shown in the lower panel. (*P < 0.01, ANOVA followed by Student–Newman–Keuls test). (D) Levels of FAP in fibroblasts were determined by immunohistochemistry staining. Scale bars, 200 μm.