Figure 3.

c‐Ski overexpression enhances the proliferation, migration, invasion and contractile activities of fibroblasts. (A, B) Cellular proliferation assays were conducted by using either (A) MTT assay or (B) flow cytometric assay for NFs, NFs stably transfected with c‐Ski (NF/c‐Ski) or control vector (NF/Vec), and parental CAFs. The data represent means ± s.d. from three different experiments. (C, D) Cell invasion or migration were tested by Transwell Chamber (C) or wound healing assay (D) for parental NFs, parental CAFs, NF/c‐Ski and its control cells (NF/Vec). (**P < 0.01, Student t test.) (E) The contractile activity of each fibroblast was checked by contraction assays in collagen gel. Representative images of collagen gels are shown. (*P < 0.05, **P < 0.01, ANOVA followed by Student–Newman–Keuls test.)