Figure 5.

Silence of c‐Ski abrogates CAFs activation and function. (A) Levels of c‐Ski, α‐SMA, FAP in CAFs stably transfected with pLVX‐shRNA1‐Ski (CAF‐sh/c‐Ski) or control vector (CAF‐sh/Vec) were determined by Western blot analysis (Left panel). β‐Actin was used as loading control. The relative fold changes of proteins levels were quantified by density value relative to the loading control (Right panel). The data represent means ± s.d. from three different experiments. (*P < 0.01, ANOVA followed by Student–Newman–Keuls test). (B) The invasion of breast cancer MDA‐MB‐231 cells was analyzed by transwell assays. CM collected from parental CAFs, parental NFs, CAFs‐sh/c‐Ski and its control cells (CAFs‐sh/Vec) were separately added into lower chambers of transwell as chemoattractants. (*P < 0.01, ANOVA followed by Student–Newman–Keuls test.)