Figure 5.

Insulin+BrdU+ beta cells in human islet grafts from alogliptin-treated mice.

Notes: Representative micrographs of human islets from a single donor (Donor 1) engrafted in (A) vehicle control or (B) alogliptin-treated mice are shown. Top panels show insulin (red) and BrdU (green) staining; bottom panels include nuclei staining with DAPI (blue). Similar results were seen with islet grafts from Donors 2 and 3. Arrows show insulin+BrdU+ cells; arrowheads indicate insulin-negative BrdU+ cells; cells were only counted as insulin+ if the staining was visible around the entire perimeter of the nucleus.

Abbreviations: BrdU, bromodeoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole.