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. 2013 Dec 16;8(12):e82294. doi: 10.1371/journal.pone.0082294

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© 2013 Liao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HMEC-1 cells (1.5×10⁵cells/well) were seeded onto Matrigel-coated 24-well plates and incubated with solvent control (0.1% DMSO) or various concentrations of tryptanthrin for 16 h. (A) Representative appearance of HMEC-1 tube formation. (B) The tube formation was quantified by counting the number of branch points of the capillary network. Data are expressed as mean ± SD. *** p<0.001.