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. 2013 Dec 16;8(12):e82563. doi: 10.1371/journal.pone.0082563

Figure 2. Nicotine-mediated LDLR up-regulation is dependent on nAChR.

Figure 2

(A) The expression of nAChR subunits was assessed by real-time PCR for Ca9-22 (upper panel) and primary cultured human gingival epithelial cells (lower panel). The expression level of GAPDH was set as 1. Relative expression level of each nAChR subunit to GAPDH was shown. (B) Ca9-22 cells were pre-treated with graded concentrations of αBtx for 1 h. The cells were washed and further incubated with 100 μM of nicotine for 3 h. LDLR expression was examined by real-time PCR. Data from at least 3 separate experiments are shown (mean ± SD). *p < 0.05.