Figure 3. Truncated FUS 1–359 is not recruited into stress granules in naïve SH-SY5Y cells. Representative confocal images of cells transfected with plasmids expressing various GFP-tagged FUS variants and stained with antibodies that detect stress granules. Foci spontaneously formed by FUS R522G or FUS 1–513 in a fraction of transfected cells are positive for TIAR (A) or G3BP1 (B), markers for stress granules, whereas neither large juxtanuclear, nor small dispersed aggregates formed by FUS 1–359 contain these proteins. (C) Emetine treatment disperses foci formed by FUS R522G, but has no effect on FUS 1–359 aggregates. Scale bars: 15 µm for all panels.