Figure 4. Truncated FUS 1–359 is not recruited into stress granules upon oxidative insult. (A) Cells transfected with GFP-tagged FUS expressing plasmids were treated with 0.5 mM sodium arsenite for 1 h and stained with anti-TIAR antibody. Foci formed by FUS R522G and FUS 1–513 are positive for TIAR, while FUS 1–359 aggregates (arrows) do not co-localize with TIAR-positive stress granules (arrowheads). (B) Cycloheximide treatment blocks formation of stress granules including those containing FUS 1–513, but does not affect FUS 1–359 aggregates. Scale bar: 15 µm for all panels.