Figure 6. Truncated FUS 1–359 protein is able to sequester both normal and mutated FUS variants in aggregates. Cells were co-transfected with shown GFP-tagged FUS variants, and stained with antibody against C terminus of FUS. Cytoplasmic variants FUS R522G and FUS 1–513 are recruited into morphologically distinct aggregates formed by FUS 1–359 (arrowheads). In some, relatively rare cells FUS 1–359 recruits into these aggregates even a fraction of co-expressed wild-type FUS or endogenous wild-type FUS retained in the cytoplasm (lower panel). Scale bar: 10 µm for all panels.