Skip to main content
. 2013 Dec 16;8(12):e82526. doi: 10.1371/journal.pone.0082526

Figure 5. Differential upregulation of BAT specific and fatty acid oxidation genes in vivo and in vitro.

Figure 5

qPCR analyses of BAT specific gene UCP1 (A); genes involved in mitochondrial β-oxidation and peroxisomal lipid oxidation: Pparα and its targeted genes Cpt1α, Acot2 and Acox2 (B) in siolated adipocytes from EWAT of Bscl2+/+ and Bscl2−/− mice. Each sample was pooled from EWAT obtained from three 6-week-old nonfasting male wild-type and Bscl2 −/− mice (n  =  4). qPCR analyses of BAT specific gene Ucp1 (C) and Elovl3 (D) in D2 (2 days after hormone cocktail addition to induce differentiation), D3, D4 and D5 differentiating Bscl2+/+ and Bscl2−/− MEFs. Data were normalized to Cyclophilin A and expressed as relative fold changes as compared to wild-type at D2. *: P<0.05; **: p<0.005.