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. 2013 Dec 16;8(12):e82526. doi: 10.1371/journal.pone.0082526

Figure 6. The residual Bscl2 − /− subcutaneous white adipose tissues were not browning but had similar altered lipid metabolism.

Figure 6

qPCR analyses of BAT specific genes Ucp1 and Elovl3, lipolytic product activated transcription factor Pparα and its targeted genes Cpt1α and Acox2 (A); and genes involved in elongation, desaturation and TG synthesis (B) in isolated adipocytes from ScWAT of Bscl2+/+ and Bscl2−/− mice. Each sample was pooled from 3-4 6-week-old nonfasting male wild-type and Bscl2 −/− mice (n  =  4–5). *: P<0.05; **: p<0.005. (C) TLC analysis of total lipids extracted from ScWAT of male non-fasting Bscl2+/+ and Bscl2−/− mice (n = 5–6). Total lipids from equal amounts of tissue for each genotype were loaded.