Table 2. Lipidomic analysis of DGs by shotgun mass spectrometry of EWAT from Bscl2+/+ and Bscl2−/− mice.
DG | Bscl2+/+ | Bscl2− /− |
DG32:5 | 0.82±0.59 | 0.19±0.06 |
DG32:4 | 0.29±0.03 | 0.09±0.02*↓ |
DG32:3 | 0±0 | 0.63±0.96 |
DG32:2 | 0.32±0.06 | 0.27±0.02 |
DG32:1 | 1.14±0.6 | 0.61±0.18 |
DG32:0 | 2.08±1.13 | 0.95±0.15 |
DG34:3 | 3.95±1.69 | 4.31±0.18 |
DG34:2 | 32.66±0.82 | 25.96±0.97*↓ |
DG34:1 | 13.73±0.66 | 15.46±1.79 |
DG36:5 | 0.28±0.13 | 0.07±0.03**↓ |
DG36:4 | 11.57±0.88 | 8.54±0.93*↓ |
DG36:3 | 23.2±1.61 | 24.99±0.59 |
DG36:2 | 9.45±0.74 | 14.97±1.61*↑ |
DG36:1 | 0.12±0.11 | 0.36±0.14**↑ |
DG38:4 | 0.12±0.13 | 1.29±0.16*↑ |
DG40:8 | 0.25±0.23 | 1.02±0.88 |
DG48:1 | 0±0 | 0.11±0.03*↑ |
DG48:0 | 0±0 | 0.12±0.05*↑ |
DG species were determined using high resolution ESI-MS and confirmed via product ion scan mode CID-MS/MS as described in Methods (n = 3 pooled from 6 animals). Data are expressed as % total DG ion abundance in each genotype. Data are presented as means ± SD. *: p<0.05; **: p<0.005. Arrows indicate upregulation or downregulation vs. Bscl+/+ EWAT.