Figure 4. Survival of Galleria mellonella larvae inoculated with mucoid isolates and nonmucoid variants of Burkholderia.
Triplicate groups of 10 larvae were inoculated with the mucoid parental isolate or the nonmucoid variants obtained during prolonged stationary phase or stress by ciprofloxacin (indicated by the symbol #). The control experiment without bacteria is also represented. Larvae were injected with approximately 1×105 Burkholderia cells with the exceptions of B. phymatum STM815 and B. stabilis LMG18888 with approximately 1×107 cells and B. cepacia IST408 and B. ambifaria CEP0958 with 1×104 cells. Survival rates were determined for 7 days. A P-value of <0.05 was considered significant compared with the respective mucoid parental isolate (*).