Table 1. Bacterial strains used in this study.
Isolate | Relevant characteristic(s) | EPS production (g/l) | Ciprofloxacin MIC (µg/ml) | Exposure [ciprofloxacin](µg/ml) | Source or reference |
B. multivorans D2095 | Cystic fibrosis clinical isolate, Canada; EPS+ (cepacian)§ | 3.7±0.1 | 4 | 10 | [13] |
B. multivorans D2214 | Cystic fibrosis clinical isolate, Canada; EPS− | 0.0 | ND | - | [13] |
B. multivorans ATCC 17616 | Soil isolate, USA; EPS+(cepacian) § | 0.3±0.1 | 1 | 2.5 | [33] |
B. cepacia IST408 | Cystic fibrosis clinical isolate, Portugal; EPS+ (cepacian) § | 1.2±0.5 | 1 | 2.5 | [34] |
B. cepacia ATCC 17759 | Soil isolate, Trinidad; EPS+# | ND | 1 | 2.5 | [35] |
B. cenocepacia J415 | Cystic fibrosis clinical isolate, UK; EPS+# | ND | 2 | 5 | [36] |
B. stabilis LMG14086 | Respirator, UK; EPS+# | ND | 1 | 2.5 | [37] |
B. stabilis LMG18888 | Human blood, Belgium; EPS+# | 0.2±0.1 | 1 | 2.5 | [38] |
B. vietnamiensis PC259 | Cystic fibrosis clinical isolate, USA; EPS+# | ND | 1 | 2.5 | [39] |
B. ambifaria AMMD | Root-colonizing bacterium, USA; EPS+ (cepacian) § | ND | 1 | 2.5 | [40] |
B. ambifaria CEP0958 | Cystic fibrosis clinical isolate, Australia; EPS+# | 3.9± 0.3 | 1 | 2.5 | [40] |
B. dolosa CEP0743 | Cystic fibrosis clinical isolate, Canada; EPS+# | 1.2±0.4 | 2 | 5 | D. P. Speert |
B. anthina J2552 | Soil isolate, UK; EPS+# | ND | 1 | 2.5 | [41] |
B. anthina FC0974 | Cystic fibrosis clinical isolate, Canada; EPS+# | 0.4±0.2 | 1 | 2.5 | D. P. Speert |
B. phymatum STM815 | Soil isolate, French Guiana; nitrogen fixation; EPS+ (cepacian) § | 1.9±0.2 | 6* | 15 | [42] |
B. xenovorans LB400 | Soil isolate, USA; degradation of polychlorinated biphenyl compounds; EPS+ (cepacian) § | 5.2±0.2 | 6* | 15 | [43] |
ND, not determined; *MIC determined at 30°C; § the EPS produced by this strain in MM medium is cepacian as determined by condensed-phase infrared spectroscopy [44]; # the nature of this EPS was not determined; EPS+, producer of exopolysaccharide; EPS−, non EPS producer.