Figure 5. Model of two spatially regulated regimes of spindle movements that govern cell shape associated spindle orientation (A) Differential regulation of three distinct movements of the spindle. (1) Spindle rotation with a directional bias: Misoriented spindles are rotated toward the interphase cells' long-axis in an LGN-dependent manner. (2) Orientation maintenance: Spindle alignment maintained near the long-axis in an LGN-independent manner. (3) Spindle oscillation along pole-to-pole axis: During orientation maintenance phase, the spindle oscillates in an LGN-dependent manner. (B) Orientation maintenance is sensitive to suppression of microtubule dynamics but not increase in microtubule length. Illustration depicts spindle behavior in the two regimes. 2ME2 disrupts orientation by perturbing the maintenance of due to suppressed microtubule dynamics. However, an increase in astral microtubule (MT) length (MCAK-depleted cells) does not perturb directional bias or orientation maintenance.