Table 1. Effect of 2ME2 treatment on the dynamic instability of microtubules in mitotic cells.
| Mode* growth rate± SD(µm min−1) | Mode shrinkage rate ± SD (µm min−1) | Per cent time growing/shortening/paused | Pause frequency (s−1) | |
|---|---|---|---|---|
| Control (DMSO) |
14.25 ± 3.74 |
14.60 ± 9.01 |
37.4/37.4/25.1 |
0.118 |
| 2ME2 treatment | 4.860 ± 2.75 | 5.224 ± 2.25 | 38.8/36.3/24.9 | 0.130 |
* Mode values were chosen since the histograms of growth and shrinkage rates were not distributed normally. To define the mode in this table, we considered values within the peaks in the histogram and then calculated the mean and SD for the modes in individual cells. List of mode values of microtubule growth and shrinkage rates, percentage time microtubules spent growing or shrinking or pausing, and frequency of pause events (every s) observed in HeLaEB1-YFP cells that were treated with DMSO or 100 nM 2ME2. Eighty-four tracks of 5 control mitotic cells and 67 tracks of 5 2ME2-treated mitotic cells were used for analysis.