Table 2. Spindle rotation rates before and after alignment along long-axis.
| Treatment | Post-align speed (deg/frame) | S.E.M. | Pre-align speed (deg/frame) | S.E.M. | ncells | Post-align frames | Pre-align frames | Pre-align speed (deg/min) | Post-align speed (deg/min) |
|---|---|---|---|---|---|---|---|---|---|
|
DMSO (Control) |
9.8986 |
0.5326 |
13.0507 |
0.7360 |
123 |
365 |
369 |
3.2626 |
2.4746 |
|
100nM 2ME2 |
8.5838 |
0.3196 |
9.1253 |
0.2953 |
100 |
827 |
1196 |
2.2813 |
2.1459 |
|
Cntrl RNAi (72 h; syn) |
9.8077 |
0.4118 |
15.0410 |
0.7714 |
142 |
531 |
430 |
3.7602 |
2.4519 |
|
LGN RNAi (72 h; syn) |
7.7368 |
0.38547 |
8.6709 |
0.4061 |
178 |
410 |
509 |
2.1677 |
1.9342 |
|
Cntrl RNAi (48 h; asy) |
11.1944 |
1.1866 |
16.0160 |
1.3021 |
66 |
150 |
183 |
4.0040 |
2.7986 |
| MCAK RNAi (48 h; asy) | 8.8873 | 0.6562 | 12.7987 | 0.7690 | 97 | 253 | 271 | 3.1996 | 2.2218 |
Speeds were calculated using rotational displacement of spindle pole positions between two consecutive frames (T1 and T2) as determined using Spindle3D. Spindle angle at T1 was used to distinguish pre-align (> 30 from long-axis) and post-align (< 30 degrees from long-axis) positions. Frame refers to four minutes intervals. Columns marked “Pre-align frames” and “Post-align frames” indicate the number of frames used to calculate speed. Speed in degrees/min was calculated by quartering degrees/frame values. SEM refers to Standard Error of Mean. ncells refers to total number of mitotic cells used for speed calculations. Cntrl RNAi, syn and asy refer to Control RNAi, synchronous cultures and asynchronous cultures, respectively. Hours (h) in column 1 refers to transfection period. List of spindle rotation speeds from time-lapse movies extracted using spindle pole positions identified by Spindle3D. Speeds were binned according to positions occupied by the spindle, either before spindle alignment along long-axis (< 30 degrees) or after alignment along long-axis (> 30 degrees).