Figure 2. Ezh2 recruitment to laser induced DNA damage is PARP dependent. U2OS cells expressing (A) GFP-Ezh2, (B) GFP-Eed, or (C) GFP-Suz12 were treated with PARP inhibitor AG14361 (5 μM) or DMSO (control) for 1 h prior to damage induction. Cells were then laser micro-irradiated and imaged over time. Quantifications (an average of 12–15 cells was plotted) of the GFP signal at the micro-irradiated regions were plotted and shown as indicated. Error bars represent SEM.